Details

Autor(en) / Beteiligte

• Wang, Tiehui
• Bird, Steve
• Koussounadis, Antonis
• Holland, Jason W
• Carrington, Allison
• Zou, Jun
• Secombes, Christopher J

Titel

Identification of a Novel IL-1 Cytokine Family Member in Teleost Fish

Ist Teil von

• The Journal of immunology (1950), 2009-07, Vol.183 (2), p.962-974

Ort / Verlag

United States: Am Assoc Immnol

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• A novel IL-1 family member (nIL-1F) has been discovered in fish, adding a further member to this cytokine family. The unique gene organization of nIL-1F, together with its location in the genome and low homology to known family members, suggests that this molecule is not homologous to known IL-1F. Nevertheless, it contains a predicted C-terminal beta-trefoil structure, an IL-1F signature region within the final exon, a potential IL-1 converting enzyme cut site, and its expression level is clearly increased following infection, or stimulation of macrophages with LPS or IL-1beta. A thrombin cut site is also present and may have functional relevance. The C-terminal recombinant protein antagonized the effects of rainbow trout rIL-1beta on inflammatory gene expression in a trout macrophage cell line, suggesting it is an IL-1beta antagonist. Modeling studies confirmed that nIL-1F has the potential to bind to the trout IL-1RI receptor protein, and may be a novel IL-1 receptor antagonist.