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Normal bone marrow (BM) contains T cells whose function and origin are poorly understood. We observed that CD8
+ T cells in BM consist chiefly of CCR7
+ L-selectin
+ central memory cells (T
CMs). Adoptively transferred T
CMs accumulated more efficiently in the BM than naive and effector T cells. Intravital microscopy (IVM) showed that T
CMs roll efficiently in BM microvessels via L-, P-, and E-selectin, whereas firm arrest required the VCAM-1/α4β1 pathway. α4β1 integrin activation did not depend on pertussis toxin (PTX)-sensitive Gαi proteins but was reduced by anti-CXCL12. In contrast, T
CM diapedesis did not require CXCL12 but was blocked by PTX. After extravasation, T
CMs displayed agile movement within BM cavities, remained viable, and mounted potent antigen-specific recall responses for at least two months. Thus, the BM functions as a major reservoir for T
CMs by providing specific recruitment signals that act in sequence to mediate the constitutive recruitment of T
CMs from the blood.