Details

Autor(en) / Beteiligte

• Kunkel, Thomas A
• Garcia-Diaz, Miguel
• Bebenek, Katarzyna
• Krahn, Joseph M
• Pedersen, Lars C

Titel

A closed conformation for the Pol λ catalytic cycle

Ist Teil von

• Nature structural & molecular biology, 2005-01, Vol.12 (1), p.97-98

Ort / Verlag

United States: Nature Publishing Group

Erscheinungsjahr

2005

Quelle

MEDLINE

Beschreibungen/Notizen

• Pol λ is a family X member believed to fill short gaps during DNA repair. Here we report crystal structures of Pol λ representing three steps in filling a single-nucleotide gap. These structures indicate that, unlike other DNA polymerases, Pol λ does not undergo large subdomain movements during catalysis, and they provide a clear characterization of the geometry and stereochemistry of the in-line nucleotidyl transfer reaction.