Details

Autor(en) / Beteiligte

• Pituch, Hanna
• Rupnik, Maja
• Obuch-Woszczatynski, Piotr
• Grubesic, Ana
• Meisel-Mikolajczyk, Felicja
• Luczak, Miroslaw

Titel

Detection of binary-toxin genes (cdtA and cdtB) among Clostridium difficile strains isolated from patients with C. difficile-associated diarrhoea (CDAD) in Poland

Ist Teil von

• Journal of medical microbiology, 2005-02, Vol.54 (2), p.143-147

Ort / Verlag

Reading: Soc General Microbiol

Erscheinungsjahr

2005

Quelle

MEDLINE

Beschreibungen/Notizen

• 1 Department of Medical Microbiology, Medical University of Warsaw, 5 Chalubinski Street, 02-004 Warsaw, Poland 2 Department of Biology, University of Ljubljana, Ljubljana, Slovenia Correspondence Hanna Pituch hanna.pituch{at}ib.amwaw.edu.pl Received June 28, 2004 Accepted August 25, 2004 Clostridium difficile A + B + and A – B + strains isolated from stool samples of patients with C. difficile -associated diarrhoea (CDAD) were selected from the University Hospital Warsaw collection. The binary-toxin genes cdtA and cdtB were detected by PCR in five of the 41 A + B + strains tested, but in none of the 17 A – B + strains tested, giving 8.6 % prevalence (5/58) of binary-toxin-positive strains. All of the strains that were positive for binary-toxin genes were grouped into toxinotype IV, suggesting that in this institution toxinotype IV might dominate among the population of C. difficile with binary-toxin genes. Abbreviations: AAD, antibiotic-associated diarrhoea; AAC, antibiotic-associated colitis; CDAD, C. difficile -associated diarrhoea; PaLoc, pathogenicity locus; PMC, pseudomembranous colitis.