Details

Autor(en) / Beteiligte

• Martin-Blondel, G.
• Gales, A.
• Bernad, J.
• Cuzin, L.
• Delobel, P.
• Barange, K.
• Izopet, J.
• Pipy, B.
• Alric, L.

Titel

Low interleukin-10 production by monocytes of patients with a self-limiting hepatitis C virus infection

Ist Teil von

• Journal of viral hepatitis, 2009-07, Vol.16 (7), p.485-491

Ort / Verlag

Oxford, UK: Blackwell Publishing Ltd

Erscheinungsjahr

2009

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Host factors seem to be crucial for the spontaneous clearance of hepatitis C virus (HCV). Monocytes play a pivotal role in innate immunity and help regulate adaptive responses. This study assesses the characteristics of monocytes from patients with self‐limiting HCV infections. We studied 35 consecutive patients [11 with a self‐limiting HCV infection, 16 chronically infected with HCV and sustained virological responders (SVR) following antiviral therapy, and eight chronically infected HCV but untreated] and eight healthy donors (HD). The production of interleukin (IL)‐10, tumour necrosis factor‐alpha (TNF‐α) and IL‐12p40 by monocytes stimulated with lipopolysaccharides(LPS) or HCV Core protein was measured by enzyme‐linked immunoassay. Monocyte surface markers were analysed by flow cytometry. LPS and Core protein triggered IL‐10 and TNF‐α production, but monocytes from self‐limiting infection patients produced significantly less IL‐10 and TNF‐α than those of SVR, chronically infected or HD (P < 0.05), while IL‐12p40 production was unchanged. This cytokine production profile did not appear to be due to expansion of the CD14+ CD16+ monocyte subset or to a classical or alternative activation monocyte profile. Monocytes from self‐limiting infection patients had more CCR7 than those from SVR or chronically infected patients (P < 0.05). Monocytes of self‐limiting infection patients appear to produce little IL‐10 and TNF‐α in response to viral or unspecific stimulation and to have a higher CCR7 expression. This profile seems to be independent to a particular monocyte subset or activation state. Low IL‐10 production may help establish an effective immune response and spontaneous HCV clearance.