FEBS letters, 2009-06, Vol.583 (12), p.1928-1932
2009
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Details
- Autor(en) / Beteiligte
- Titel
- SSO1450 – A CAS1 protein from Sulfolobus solfataricus P2 with high affinity for RNA and DNA
- Ist Teil von
- FEBS letters, 2009-06, Vol.583 (12), p.1928-1932
- Ort / Verlag
- England: Elsevier B.V
- Erscheinungsjahr
- 2009
- Quelle
- Wiley-Blackwell Journals
- Beschreibungen/Notizen
- Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated protein genes ( cas genes) are ubiquitous in archaea and eubacteria. It has been suggested that CRISPR and CAS proteins act as an immune system preventing the invasion of foreign genomic elements at the DNA level. The protein SSO1450 from Sulfolobus solfataricus ( Sso) P2 belongs to the CAS1 cluster which is one of the core protein clusters most frequently associated with CRISPR sequences. In this study we show that SSO1450 is a high-affinity nucleic acid binding protein. It binds DNA, RNA and DNA–RNA hybrid apparently sequence non-specific in a multi-site binding mode. Furthermore, SSO1450 promotes the hybridization of complementary nucleic acid strands.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/j.febslet.2009.04.047Titel-ID: cdi_proquest_miscellaneous_67376037
- Format
- –
- Schlagworte
- apparent dissociation constant, Archaea, Archaeal Proteins - chemistry, Archaeal Proteins - genetics, Archaeal Proteins - metabolism, base pair, Base Sequence, CAS, CASS, Chromosome Mapping, cluster of orthologous groups, clustered regularly interspaced short palindromic repeats, COG, CRISPR, CRISPR-associated, CRISPR-associated system, Dimerization, dithiothreitol, DNA Primers - genetics, DNA, Archaeal - chemistry, DNA, Archaeal - genetics, DNA, Archaeal - metabolism, DNA-Binding Proteins - chemistry, DNA-Binding Proteins - genetics, DNA-Binding Proteins - metabolism, DTT, electrophoretic mobility shift assay, EMSA, Eubacteria, Fluorescence Polarization, Genes, Archaeal, Inverted Repeat Sequences, IPTG, isopropyl-β-d-thiogalactoside, K d, Luria-Bertani medium, Multigene Family, Nucleic acid binding, Nucleic Acid Hybridization, nucleotide, OD600, optical density at 600 nm, PAGE, polyacrylamide gel electrophoresis, Protein Structure, Quaternary, RNA interference, RNA, Archaeal - chemistry, RNA, Archaeal - genetics, RNA, Archaeal - metabolism, RNA-Binding Proteins - chemistry, RNA-Binding Proteins - genetics, RNA-Binding Proteins - metabolism, RNAi, SDS, single/double-stranded, sodium dodecylsulfate, ss/ds, Sso, SSO1450, Sulfolobus solfataricus, Sulfolobus solfataricus - genetics, Sulfolobus solfataricus - metabolism