Details

Autor(en) / Beteiligte

• Endharti, Agustina Tri
• Zhou, Yan Wen
• Nakashima, Izumi
• Suzuki, Haruhiko

Titel

Galectin‐1 supports survival of naive T cells without promoting cell proliferation

Ist Teil von

• European journal of immunology, 2005-01, Vol.35 (1), p.86-97

Ort / Verlag

Weinheim: WILEY‐VCH Verlag

Erscheinungsjahr

2005

Quelle

Wiley HSS Collection

Beschreibungen/Notizen

• Naive T cells do not proliferate but remain alive in vivo. In contrast, naive T cells rapidly die in an in vitro culture, suggesting that some factors that are present at the sites of naive T cell circulation in vivo but missing in the bovine serum‐containing culture medium, are necessary for their survival. The present study was designed to search for such factors. By functional screening of the cDNA library from murine lymph node‐derived stromal cells (LNS) that effectively support the survival of naive T cells, we found that nascent polypeptide‐associated complex (α−NAC) promoted T cell survival. A conditioned medium derived from culture supernatant of Cos7 cells transfected with α−NAC gene supported T cell survival, indicating that α−NAC induced production of soluble factor(s) that were secreted into the medium. By examining the products that were cloned from a functional screening of the cDNA library from α−NAC‐transfected NIH3T3 cells but were not detected in that from control vector‐transfected cells, galectin‐1 was found as a soluble factor in the conditioned medium of the LNS. Our study demonstrates the novel role of galectin‐1 as a soluble factor that functions to maintain naive T cell survival without inducing cell proliferation.