Journal of virological methods, 2005, Vol.123 (1), p.1-7
2005
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- Autor(en) / Beteiligte
- Titel
- Real-time RT-PCR for norovirus screening in shellfish
- Ist Teil von
- Journal of virological methods, 2005, Vol.123 (1), p.1-7
- Ort / Verlag
- London: Elsevier B.V
- Erscheinungsjahr
- 2005
- Quelle
- Access via ScienceDirect (Elsevier)
- Beschreibungen/Notizen
- Real-time RT-PCR, combining amplification and detection of virus-specific amplicons, is a promising tool for norovirus detection in environmental or food samples such as shellfish. We developed a real-time RT-PCR assay based on one-step detection using single primer sets and probes for norovirus genogroups I and II. Seventy and seven RT-PCR units of genogroup I and II reference norovirus strains, respectively, were detected in artificially contaminated oysters. Validation of the new method on 150 archived naturally contaminated shellfish confirmed the utility of the genogroup II primer set to detect a large range of different strains circulating in France since 1995, but genogroup I strains were detected infrequently.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0166-0934eISSN: 1879-0984DOI: 10.1016/j.jviromet.2004.08.023Titel-ID: cdi_proquest_miscellaneous_67336862
- Format
- –
- Schlagworte
- Animals, Biological and medical sciences, Classic detection, DNA Primers, Fundamental and applied biological sciences. Psychology, Microbiology, Norovirus, Norovirus - classification, Norovirus - genetics, Norovirus - isolation & purification, Ostreidae - virology, Real-time RT-PCR, Reverse Transcriptase Polymerase Chain Reaction, RNA, Viral - analysis, RNA, Viral - isolation & purification, Sensitivity and Specificity, Shellfish, Shellfish - virology, Techniques used in virology, Virology