Journal of microbiological methods, 2009-06, Vol.77 (3), p.326-329
2009
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- Autor(en) / Beteiligte
- Titel
- Rapid and accurate typing of Bordetella pertussis targeting genes encoding acellular vaccine antigens using real time PCR and High Resolution Melt analysis
- Ist Teil von
- Journal of microbiological methods, 2009-06, Vol.77 (3), p.326-329
- Ort / Verlag
- Amsterdam: Elsevier B.V
- Erscheinungsjahr
- 2009
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Real Time-PCR (RT-PCR) and high resolution melt (HRM) analyses were used for rapid typing of genes encoding components of the pertussis acellular vaccine, namely prn, ptxA, fhaB, fim2 and fim3. The length polymorphisms in prn were detected by RT-PCR followed by HRM; single nucleotide polymorphisms in prn and other genes were detected by hairpin primer RT-PCR. These rapid methods are suitable for large-scale studies of vaccine-driven evolution of Bordetella pertussis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0167-7012eISSN: 1872-8359DOI: 10.1016/j.mimet.2009.03.007Titel-ID: cdi_proquest_miscellaneous_67290551
- Format
- –
- Schlagworte
- Antigenic variation, Antigens, Bacterial - genetics, Antigens, Bacterial - immunology, Bacterial Typing Techniques - methods, Bacteriological methods and techniques used in bacteriology, Bacteriology, Biological and medical sciences, Bordetella pertussis, Bordetella pertussis - classification, Bordetella pertussis - genetics, Bordetella pertussis - immunology, Fundamental and applied biological sciences. Psychology, High resolution melt analysis, Humans, Length polymorphism, Microbiology, Pertussis Vaccine - genetics, Pertussis Vaccine - immunology, Polymerase Chain Reaction - methods, Polymorphism, Single Nucleotide, Single nucleotide polymorphism, Transition Temperature, Vaccines, Acellular - genetics, Vaccines, Acellular - immunology, Whooping cough, Whooping Cough - microbiology