Details

Autor(en) / Beteiligte

• Wang, Zhao-jun
• Zhang, Fu-min
• Wang, Lian-sheng
• Yao, Yong-wei
• Zhao, Qiang
• Gao, Xiang

Titel

Lipopolysaccharides can protect mesenchymal stem cells (MSCs) from oxidative stress-induced apoptosis and enhance proliferation of MSCs via Toll-like receptor(TLR)-4 and PI3K/Akt

Ist Teil von

• Cell biology international, 2009-06, Vol.33 (6), p.665-674

Ort / Verlag

Oxford, UK: Elsevier Ltd

Erscheinungsjahr

2009

Quelle

Wiley-Blackwell Full Collection

Beschreibungen/Notizen

• Apoptosis of implanted mesenchymal stem cells (MSCs) limits the efficiency of MSC therapy. Recent studies showed the ligands of Toll-like receptors (TLRs) could control the function of these cells. We have investigated the effect of lipopolysaccharides (LPS), a ligand of TLR4, on the survival of MSCs and explored the roles of TLR4 and PI3K/Akt. H 2O 2/serum deprivation(H 2O 2/SD) induced apoptosis of MSCs but LPS-preconditioning (1.0 μg/ml) protected MSCs from H 2O 2/SD-induced apoptosis and promoted their proliferation. Western blotting showed that 1.0 μg/ml LPS enhanced phosphorylation of both Akt at Ser 473 and nuclear factor-kappa B (NF-κB) p65 at Ser 536. However, the protective effects of LPS on survival were not observed in TLR4 lps-del MSCs. The results suggest appropriate treatments with LPS can protect MSCs from oxidative stress-induced apoptosis and improve the survival of MSCs via the TLR4 and PI3K/Akt pathway.