In vitro cellular & developmental biology. Animal, 2004-11, Vol.40 (10), p.331-336
2004
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Details
- Autor(en) / Beteiligte
- Titel
- INTERLEUKIN-8 CAN STIMULATE PROGESTERONE SECRETION FROM A HUMAN TROPHOBLAST CELL LINE, BEWO
- Ist Teil von
- In vitro cellular & developmental biology. Animal, 2004-11, Vol.40 (10), p.331-336
- Ort / Verlag
- Germany: Society for In Vitro Biology
- Erscheinungsjahr
- 2004
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Precise paracrine cross-talk between the embryo and the endometrium is essential for the establishment of a successful pregnancy. Previous studies have demonstrated that the expression of interleukin-8 (IL-8) in the endometrium is enhanced during the late-secretory phase and early pregnancy. Furthermore, IL-8 receptor (IL-8R) expression has been detected in trophoblast cells of the developing embryo. To clarify the roles of IL-8 in the endometrium–embryo interactions, the effects of IL-8 on hormone secretion by trophoblast cells were studied using the BeWo trophoblast cell line that retains hormone-secreting properties of normal trophoblast cells. Using reverse transcription–polymerase chain reaction, we found that IL-8R messenger ribonucleic acid (mRNA) was expressed in BeWo cells. The levels of IL-8R mRNA and protein expression in BeWo cells were similar to those in primary first-trimester trophoblast cells. Progesterone (P4) secretion of BeWo cells was comparable with that of first-trimester trophoblast cells but higher than that of third-trimester trophoblast cells. Treatment of BeWo cells with recombinant human IL-8 (rhIL-8) had no effect on cell proliferation, as determined by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Interestingly, secretion of P4, but not human chorionic gonadotropin, from cultured BeWo cells was significantly enhanced when the cells were incubated with rhIL-8. Our results demonstrate that IL-8 may play an important role in the endometrium–embryo interactions by stimulating trophoblast secretion of P4 for maintenance of a successful pregnancy.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1071-2690, 1543-706XeISSN: 1543-706XDOI: 10.1290/0404033.1Titel-ID: cdi_proquest_miscellaneous_67270151
- Format
- –
- Schlagworte
- Cell culture techniques, Cell growth, Cell Line, Tumor, Cell lines, Cell Proliferation - drug effects, Cultured cells, CYTOKINES/GROWTH FACTORS/ADHESION FACTORS, DNA Primers, embryo implantation, Endometrium - cytology, Endometrium - metabolism, Endothelial cells, Epithelial cells, Female, Flow Cytometry, Fluorescent Antibody Technique, hCG, Humans, IL-8, Interleukin-8 - pharmacology, Messenger RNA, P4, Pregnancy, Progesterone - secretion, Radioimmunoassay, Receptors, Interleukin-8B - metabolism, Recombinant Proteins - metabolism, Recombinant Proteins - pharmacology, Reverse Transcriptase Polymerase Chain Reaction, RNA, RNA, Messenger - metabolism, Secretion, Tetrazolium Salts, Thiazoles, trophectoderm, Trophoblasts, Trophoblasts - secretion