Bioresource technology, 2005-03, Vol.96 (4), p.429-436
2005
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- Autor(en) / Beteiligte
- Titel
- Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K
- Ist Teil von
- Bioresource technology, 2005-03, Vol.96 (4), p.429-436
- Ort / Verlag
- Oxford: Elsevier Ltd
- Erscheinungsjahr
- 2005
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The physical factors affecting the production of an organic solvent-tolerant protease from Pseudomonas aeruginosa strain K was investigated. Growth and protease production were detected from 37 to 45 °C with 37 °C being the optimum temperature for P. aeruginosa. Maximum enzyme activity was achieved at static conditions with 4.0% (v/v) inoculum. Shifting the culture from stationary to shaking condition decreased the protease production (6.0–10.0% v/v). Extracellular organic solvent-tolerant protease was detected over a broad pH range from 6.0 to 9.0. However, the highest yield of protease was observed at pH 7.0. Neutral media increased the protease production compared to acidic or alkaline media
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0960-8524eISSN: 1873-2976DOI: 10.1016/j.biortech.2004.06.012Titel-ID: cdi_proquest_miscellaneous_67246803
- Format
- –
- Schlagworte
- Biological and medical sciences, Bioreactors - microbiology, Biotechnology, Cell Culture Techniques - methods, Cell Proliferation, Drug Tolerance, Enzyme Activation, Enzyme engineering, Enzyme Stability, Fermentation, Fundamental and applied biological sciences. Psychology, Hydrogen-Ion Concentration, Methods. Procedures. Technologies, Motion, Organic Chemicals - chemistry, Organic solvent-tolerant protease, Peptide Hydrolases - biosynthesis, Peptide Hydrolases - chemistry, Peptide Hydrolases - classification, Physical factors, Production of selected enzymes, Pseudomonas aeruginosa, Pseudomonas aeruginosa - classification, Pseudomonas aeruginosa - enzymology, Solvents - chemistry, Species Specificity, Temperature