Details

Autor(en) / Beteiligte

• Fujihira, Takuma
• Kishida, Rie
• Fukui, Yutaka

Titel

Developmental capacity of vitrified immature porcine oocytes following ICSI: effects of cytochalasin B and cryoprotectants

Ist Teil von

• Cryobiology, 2004-12, Vol.49 (3), p.286-290

Ort / Verlag

Netherlands: Elsevier Inc

Erscheinungsjahr

2004

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• In the present study, effects of concentration and pretreatment time of cytochalasin B (CB), and of two types of cryoprotectant solutions on the nuclear maturation of vitrified–warmed porcine oocytes were examined. Also, the developmental capacity of vitrified immature porcine oocytes following intracytoplasmic sperm injection (ICSI) was investigated. The nuclear maturation rate (46.8%) of the vitrified–warmed oocytes treated with 7.5 μg/mL CB for 30 min was significantly higher ( P < 0.05) than those (13.9–39.2%) of the vitrified–warmed oocytes treated with 0, 2.5, or 5.0 μg/mL CB for 10 or 30 min. Additionally, the nuclear maturation rate of oocytes treated with CB and vitrified in ethylene glycol (EG) (37.1%) was significantly higher ( P < 0.05) than that of EG + dimethyl sulfoxide (Me 2SO) (23.9%). However, no significant differences were observed in the cleavage and blastocyst development rates among the control (45.2 and 20.0%, respectively), the EG group (37.8 and 13.5%, respectively) and the EG + Me 2SO group (39.3 and 14.3%, respectively). These results demonstrated that: (1) pretreatment with 7.5 μg/mL CB was beneficial for the vitrification of immature porcine oocytes; (2) the combination of EG and Me 2SO as a cryoprotectant was not advantageous for in vitro maturation (IVM) of vitrified immature porcine oocytes; and (3) vitrified–warmed porcine oocytes matured after IVM, developed to the blastocyst stage without distinct differences compared to fresh oocytes following ICSI.