Journal of cancer research and clinical oncology, 2009-06, Vol.135 (6), p.829-835
2009
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- Autor(en) / Beteiligte
- Titel
- Inhibitor of growth 4 induces apoptosis in human lung adenocarcinoma cell line A549 via Bcl-2 family proteins and mitochondria apoptosis pathway
- Ist Teil von
- Journal of cancer research and clinical oncology, 2009-06, Vol.135 (6), p.829-835
- Ort / Verlag
- Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag
- Erscheinungsjahr
- 2009
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Objective Inhibitor of growth 4 (ING4) is considered to be a tumor suppressor implicated in several human malignancies by tumor growth inhibition and apoptosis enhancement. In present study, the effects of ING4 on apoptosis and its mechanisms were investigated through the transduction of ING4 cDNA into lung adenocarcinoma cell line A549. Methods The effects of ING4 on A549 apoptosis were observed by FCM analysis, TUNEL assay, and electron microscopy. Simultaneously, the effects of ING4 on the expression of several apoptosis-related proteins in cell line A549 were evaluated by Western blot analysis. Results Both Annexin-V FITC analysis by FCM and TUNEL assay revealed more apoptotic cells in A549 cells with exogenous ING4 gene. For electron microscopy, A549 cells with exogenous ING4 gene showed typical morphological changes of apoptosis. The deregulation of Bcl-2 family proteins (Bcl-2, Bcl-xl, Bax, Bak, Bid) and the major apoptotic executioners of mitochondria pathway (Cyt-c, caspase3, PARP) were also observed. Conclusion Our findings suggest that exogenous ING4 can enhance A549 apoptosis via regulating the expression of Bcl-2 family proteins and the activation of mitochondrial apoptotic pathway.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0171-5216eISSN: 1432-1335DOI: 10.1007/s00432-008-0519-7Titel-ID: cdi_proquest_miscellaneous_67179779
- Format
- –
- Schlagworte
- Adenocarcinoma - metabolism, Adenocarcinoma - pathology, Adenocarcinoma - physiopathology, Animals, Antineoplastic agents, Apoptosis, Apoptosis - physiology, Biological and medical sciences, Blotting, Western, Cancer Research, Caspase 3 - metabolism, Cell Cycle Proteins - genetics, Cell Cycle Proteins - metabolism, Cell Cycle Proteins - physiology, Cell Line, Tumor, Cytochromes c - metabolism, Flow Cytometry, Gene expression, Hematology, Homeodomain Proteins - genetics, Homeodomain Proteins - metabolism, Homeodomain Proteins - physiology, Humans, Immunohistochemistry, In Situ Nick-End Labeling, Internal Medicine, Lung - metabolism, Lung - pathology, Lung - ultrastructure, Lung cancer, Lung Neoplasms - metabolism, Lung Neoplasms - pathology, Lung Neoplasms - physiopathology, Male, Medical sciences, Medicine, Medicine & Public Health, Mice, Mice, Inbred BALB C, Mice, Nude, Microscopy, Electron, Mitochondria, Mitochondria - metabolism, Mitochondrial DNA, Neoplasm Transplantation, Oncology, Original Paper, Pharmacology. Drug treatments, Pneumology, Proteins, Proto-Oncogene Proteins c-bcl-2 - metabolism, Signal transduction, Signal Transduction - physiology, Transfection, Transplantation, Heterologous, Tumor Suppressor Proteins - genetics, Tumor Suppressor Proteins - metabolism, Tumor Suppressor Proteins - physiology, Tumors of the respiratory system and mediastinum