Details

Autor(en) / Beteiligte

• Lanigan, Peter M P
• Ninkovic, Tanja
• Chan, Karen
• de Mello, Andrew J
• Willison, Keith R
• Klug, David R
• Templer, Richard H
• Neil, Mark A A
• Ces, Oscar

Titel

A microfluidic platform for probing single cell plasma membranes using optically trapped Smart Droplet Microtools (SDMs)

Ist Teil von

• Lab on a chip, 2009-01, Vol.9 (8), p.1096-1101

Ort / Verlag

England

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• We recently introduced a novel platform based upon optically trapped lipid coated oil droplets (Smart Droplet Microtools-SDMs) that were able to form membrane tethers upon fusion with the plasma membrane of single cells. Material transfer from the plasma membrane to the droplet via the tether was seen to occur. Here we present a customised version of the SDM approach based upon detergent coated droplets deployed within a microfluidic format. These droplets are able to differentially solubilise the plasma membrane of single cells with spatial selectivity and without forming membrane tethers. The microfluidic format facilitates separation of the target cells from the bulk SDM population and from downstream analysis modules. Material transfer from the cell to the SDM was monitored by tracking membrane localized EGFP.