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Early treatment with Lactobacillus delbrueckii strain induces an increase in intestinal T-cells and granulocytes and modulates immune-related genes of larval Dicentrarchus labrax (L.)
Ist Teil von
Fish & shellfish immunology, 2009-03, Vol.26 (3), p.368-376
Ort / Verlag
England: Elsevier Ltd
Erscheinungsjahr
2009
Link zum Volltext
Quelle
Access via ScienceDirect (Elsevier)
Beschreibungen/Notizen
Lactobacillus delbrueckii ssp.
delbrueckii (AS13B), isolated from the gut of adult
Dicentrarchus labrax, was administered live to developing sea bass using rotifers and
Artemia as live carriers. Immune-related gene transcripts were quantified in post-larvae at day 70 post-hatch (ph) and histology, electron microscopy and immunocytochemistry of the intestinal tissue were performed at day 74 ph. Since the probiotic was orally administered the studies were focused on intestinal immunity.
In treated fish gut integrity was unaffected, while the density of T-cells and acidophilic granulocytes in the intestinal mucosa was significantly higher than in controls. Probiotic-induced increases in intestinal T-cells and total body
TcR-β transcripts are first reported in fish. Significantly lower
IL-1β transcripts and a trend towards lower
IL-10,
Cox-2 and
TGF-β transcription were found in the treated group.
Evidence is provided that early feeding with probiotic-supplemented diet stimulated the larval gut immune system and lowered transcription of key pro-inflammatory genes.