Bioconjugate chemistry, 2004-11, Vol.15 (6), p.1403-1407
2004
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Details
- Autor(en) / Beteiligte
- Titel
- Developing a Peptide-Based Near-Infrared Molecular Probe for Protease Sensing
- Ist Teil von
- Bioconjugate chemistry, 2004-11, Vol.15 (6), p.1403-1407
- Ort / Verlag
- United States: American Chemical Society
- Erscheinungsjahr
- 2004
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Recently near-infrared (NIR) molecular probes have become important reporter molecules for a number of types of in vivo biomedical imaging. A peptide-based NIR fluorescence probe consisting of a NIR fluorescence emitter (Cy5.5), a NIR fluorescence absorber (NIRQ820), and a protease selective peptide sequence was designed to sense protease activity. Using a MMP-7 model, we showed that NIRQ820 efficiently absorbs the emission energy of Cy5.5 resulting in a low initial signal. Upon reacting with its target, MMP-7, the fluorescence signal of the designed probe was increased by 7-fold with a K cat/K m of 100 000 M-1 s-1. The described synthetic strategy should have wide application for other NIR probe preparations.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1043-1802eISSN: 1520-4812DOI: 10.1021/bc049924sTitel-ID: cdi_proquest_miscellaneous_67081100
- Format
- –
- Schlagworte
- Animals, Biomedical research, Cell Line, Tumor, Enzymes, Humans, Matrix Metalloproteinase 7, Medical imaging, Metalloendopeptidases - metabolism, Mice, Mice, Nude, Molecular Probes - metabolism, Molecules, Peptide Fragments - chemistry, Peptide Fragments - metabolism, Peptide Hydrolases - metabolism, Peptides, Spectroscopy, Near-Infrared - methods