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Na+/H+ Exchanger-3 is involved in mouse blastocyst formation
Journal of experimental zoology. Part A, Comparative experimental biology, 2004-09, Vol.301A (9), p.767-775
Kawagishi, Rikako
Tahara, Masahiro
Sawada, Kenjiro
Morishige, Kenichiro
Sakata, Masahiro
Tasaka, Keiichi
Murata, Yuji
2004
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Kawagishi, Rikako
Tahara, Masahiro
Sawada, Kenjiro
Morishige, Kenichiro
Sakata, Masahiro
Tasaka, Keiichi
Murata, Yuji
Titel
Na+/H+ Exchanger-3 is involved in mouse blastocyst formation
Ist Teil von
Journal of experimental zoology. Part A, Comparative experimental biology, 2004-09, Vol.301A (9), p.767-775
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2004
Quelle
MEDLINE
Beschreibungen/Notizen
The mouse blastocyst consists of the trophectoderm, the inner cell mass, and a fluid‐filled cavity, the blastocoel. Formation and subsequent expansion of this cavity is important for further differentiation of the inner cell mass and successful implantation. Previous work provided evidence that vectorial transport of Na+ and Cl− ions through the trophectoderm into the blastocoel generates an osmotic gradient that drives fluid across this epithelium. As the activity of the Na+/H+ exchanger (NHE) has been implicated as the exchanger responsible for facilitating the transtrophectodermal Na+ flux, the functional role of NHE in mouse blastocoel development was determined. Embryos were cultured in the presence of subtype‐specific NHE inhibitors to examine the role of NHEs in blastocoel development. When 2‐cell stage embryos were treated continuously with a specific inhibitor of NHE‐1, cariporide, the embryos passed beyond the 8‐cell stage and became blastocysts. However, in the presence of a specific inhibitor of NHE‐3, S3226, the 2‐cell stage embryos developed to the morula stage but formation of the blastocyst were inhibited in a dose‐dependent manner. Cariporide did not inhibit the formation of the blastocoel cavity from the morula stage whereas S3226 did inhibit that process. S3226 also reduced the rate of re‐expansion of blastocysts collapsed by cytochalasin D upon transfer to the control medium. An immunofluorescence study showed that NHE‐3 was detected in the vicinity of the cell membrane of the trophectoderm, especially in the apical cell margins of the trophectoderm. These results suggest that NHE‐3 is likely involved in blastocyst formation. J. Exp. Zool. 301A:767–775, 2004. © 2004 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 1548-8969
eISSN: 1552-499X
DOI: 10.1002/jez.a.90
Titel-ID: cdi_proquest_miscellaneous_67076568
Format
–
Schlagworte
Analysis of Variance
,
Animals
,
Blastocyst - drug effects
,
Blastocyst - metabolism
,
Blastocyst - physiology
,
Dose-Response Relationship, Drug
,
Fluorescent Antibody Technique
,
Guanidines - pharmacology
,
Immunohistochemistry
,
Methacrylates - pharmacology
,
Mice
,
Microscopy, Confocal
,
Sodium-Hydrogen Exchangers - antagonists & inhibitors
,
Sodium-Hydrogen Exchangers - metabolism
,
Sulfones - pharmacology
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