Nature (London), 2009-03, Vol.458 (7236), p.357-361
2009
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- Autor(en) / Beteiligte
- Titel
- Defensin-like polypeptide LUREs are pollen tube attractants secreted from synergid cells
- Ist Teil von
- Nature (London), 2009-03, Vol.458 (7236), p.357-361
- Ort / Verlag
- London: Nature Publishing Group UK
- Erscheinungsjahr
- 2009
- Quelle
- EBSCOhost Psychology and Behavioral Sciences Collection
- Beschreibungen/Notizen
- For more than 140 years, pollen tube guidance in flowering plants has been thought to be mediated by chemoattractants derived from target ovules. However, there has been no convincing evidence of any particular molecule being the true attractant that actually controls the navigation of pollen tubes towards ovules. Emerging data indicate that two synergid cells on the side of the egg cell emit a diffusible, species-specific signal to attract the pollen tube at the last step of pollen tube guidance. Here we report that secreted, cysteine-rich polypeptides (CRPs) in a subgroup of defensin-like proteins are attractants derived from the synergid cells. We isolated synergid cells of Torenia fournieri, a unique plant with a protruding embryo sac, to identify transcripts encoding secreted proteins as candidate molecules for the chemoattractant(s). We found two CRPs, abundantly and predominantly expressed in the synergid cell, which are secreted to the surface of the egg apparatus. Moreover, they showed activity in vitro to attract competent pollen tubes of their own species and were named as LUREs. Injection of morpholino antisense oligomers against the LUREs impaired pollen tube attraction, supporting the finding that LUREs are the attractants derived from the synergid cells of T. fournieri.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0028-0836eISSN: 1476-4687DOI: 10.1038/nature07882Titel-ID: cdi_proquest_miscellaneous_67048993
- Format
- –
- Schlagworte
- Agronomy. Soil science and plant productions, Amino Acid Sequence, Attractants, Biological and medical sciences, Botany, Cells, Chemotactic Factors - chemistry, Chemotactic Factors - metabolism, Chemotactic Factors - pharmacology, Chemotactic Factors - secretion, cysteine-rich polypeptides, Defensins - chemistry, Defensins - metabolism, Defensins - pharmacology, Defensins - secretion, DNA binding proteins, E coli, Economic plant physiology, embryo sac, Expressed Sequence Tags, fertilization (reproduction), Fertilization of plants, Flowering plants, Flowering shrubs, Flowering, floral biology, reproduction patterns, Fundamental and applied biological sciences. Psychology, gene expression, Genetic aspects, Growth and development, Humanities and Social Sciences, letter, Magnoliopsida - cytology, Magnoliopsida - drug effects, Magnoliopsida - genetics, Magnoliopsida - growth & development, messenger RNA, Molecular Sequence Data, multidisciplinary, Oligonucleotides, Antisense - genetics, ovules, Physiological aspects, Plant cells and tissues, Pollen, Pollen tube, Pollen Tube - drug effects, Pollen Tube - genetics, Pollen Tube - growth & development, pollen tubes, pollination, Polypeptides, Properties, Proteins, RNA, Plant - antagonists & inhibitors, RNA, Plant - genetics, RNA, Plant - metabolism, Science, Science (multidisciplinary), synergid cells, Torenia fournieri, Transcription, Genetic