Nature chemical biology, 2009-04, Vol.5 (4), p.206-215
2009
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Details
- Autor(en) / Beteiligte
- Titel
- Emerging methods for the production of homogeneous human glycoproteins
- Ist Teil von
- Nature chemical biology, 2009-04, Vol.5 (4), p.206-215
- Ort / Verlag
- New York: Nature Publishing Group US
- Erscheinungsjahr
- 2009
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Most circulating human proteins exist as heterogeneously glycosylated variants (glycoforms) of an otherwise homogeneous polypeptide. Though glycan heterogeneity is most likely important to glycoprotein function, the preparation of homogeneous glycoforms is important both for the study of the consequences of glycosylation and for therapeutic purposes. This review details selected approaches to the production of homogeneous human N- and O-linked glycoproteins with human-type glycans. Particular emphasis is placed on recent developments in the engineering of glycosylation pathways within yeast and bacteria for in vivo production, and on the in vitro remodeling of glycoproteins by enzymatic means. The future of this field is very exciting.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1552-4450eISSN: 1552-4469DOI: 10.1038/nchembio.148Titel-ID: cdi_proquest_miscellaneous_67044116
- Format
- –
- Schlagworte
- Bacteria - genetics, Biochemical Engineering, Biochemistry, Biomedical research, Bioorganic Chemistry, Cell Biology, Chemical reactions, Chemical synthesis, Chemistry, Chemistry and Materials Science, Chemistry/Food Science, Gene Expression Regulation, Genetic Engineering, Glycoproteins, Glycoproteins - chemical synthesis, Glycoproteins - genetics, Glycoproteins - metabolism, Heterogeneity, Humans, review-article, Saccharomyces cerevisiae - genetics, Yeasts