Details

Autor(en) / Beteiligte

• PASCU, Mihail Lucian
• STAICU, Angela
• VOICU, Letitia
• BREZEANU, Mihail
• CARSTOCEA, Benone
• PASCU, Ruxandra
• GAZDARU, Doina

Titel

Methotrexate as a Photosensitiser

Ist Teil von

• Anticancer research, 2004-09, Vol.24 (5A), p.2925-2930

Ort / Verlag

Attiki: International Institute of Anticancer Research

Erscheinungsjahr

2004

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• The absorption, excitation and emission spectra of methotrexate (MTX) solutions in natural saline water and sodium hydroxide at 10 -4 -10 -5 M pH=8.4 were measured, while they were exposed to coherent and uncoherent visible and near ultraviolet (UV) light. Xe and Hg lamp and also a nitrogen pulsed laser radiation were used. The absorption spectra exhibited spectral bands between 225nm and 450nm. The 200nm - 500nm excitation spectra were measured with emission centred on 470nm; fluorescence excitation was observed at 340nm and 370nm; emission fluorescence was detected between 400nm and 600nm, showing a maximum at 470nm. Spectra modifications, non-linearly depending on exposure time (varying from 3min to 180min), indicated the photodissociation of MTX to the fluorescent compound 2,4 diamino-formylpteridine. In a preliminary in vivo experiment, rapid destruction of neovascularisation was observed in the MTX-impregnated conjunctive of the rabbit eye exposed to uncoherent UV and visible light.