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Attiki: International Institute of Anticancer Research
Erscheinungsjahr
2004
Quelle
MEDLINE
Beschreibungen/Notizen
The absorption, excitation and emission spectra of methotrexate (MTX) solutions in natural saline water and sodium hydroxide
at 10 -4 -10 -5 M pH=8.4 were measured, while they were exposed to coherent and uncoherent visible and near ultraviolet (UV) light. Xe and
Hg lamp and also a nitrogen pulsed laser radiation were used. The absorption spectra exhibited spectral bands between 225nm
and 450nm. The 200nm - 500nm excitation spectra were measured with emission centred on 470nm; fluorescence excitation was
observed at 340nm and 370nm; emission fluorescence was detected between 400nm and 600nm, showing a maximum at 470nm. Spectra
modifications, non-linearly depending on exposure time (varying from 3min to 180min), indicated the photodissociation of MTX
to the fluorescent compound 2,4 diamino-formylpteridine. In a preliminary in vivo experiment, rapid destruction of neovascularisation
was observed in the MTX-impregnated conjunctive of the rabbit eye exposed to uncoherent UV and visible light.