Details

Autor(en) / Beteiligte

• Gutwein, Paul
• Schramme, Anja
• Voss, Beren
• Abdel-Bakky, Mohamed Sadek
• Doberstein, Kai
• Ludwig, Andreas
• Altevogt, Peter
• Hansmann, Martin-Leo
• Moch, Holger
• Kristiansen, Glen
• Pfeilschifter, Josef

Titel

Downregulation of junctional adhesion molecule-A is involved in the progression of clear cell renal cell carcinoma

Ist Teil von

• Biochemical and biophysical research communications, 2009-03, Vol.380 (2), p.387-391

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• Junctional adhesion molecule-A (JAM-A) is one component of tight junctions which are involved in important processes like paracellular permeability, cell polarity, adhesion, migration, and angiogenesis. Here we describe JAM-A expression in distal convoluted tubule, connecting tubule, and in cells of the collecting duct of the healthy human kidney. In addition, JAM-A was weakly expressed in cells of the proximal tubule. Using immunofluorescence, FACS and Western blot analysis we investigated JAM-A expression in tubular cells in vitro. Interestingly, treatment of HK-2 cells with IFN-γ and TNF-α resulted in a metalloproteinase mediated downregulation of JAM-A. Importantly, in a tissue micro-array JAM-A protein expression was significantly downregulated in patients with clear cell renal cell carcinoma. Furthermore, knockdown of JAM-A with JAM-A specific siRNA induced the migration of RCC4 cells. In summary, downregulation of JAM-A is an early event in the development of renal cancer and increases the migration of renal cancer cells.