Details

Autor(en) / Beteiligte

• Costa, Rossana M.
• Magalhães, Ana S.
• Pereira, José A.
• Andrade, Paula B.
• Valentão, Patrícia
• Carvalho, Márcia
• Silva, Branca M.

Titel

Evaluation of free radical-scavenging and antihemolytic activities of quince ( Cydonia oblonga) leaf: A comparative study with green tea ( Camellia sinensis)

Ist Teil von

• Food and chemical toxicology, 2009-04, Vol.47 (4), p.860-865

Ort / Verlag

Oxford: Elsevier Ltd

Erscheinungsjahr

2009

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• This study aimed to determine the phenolic profile and to investigate the antioxidant potential of quince ( Cydonia oblonga) leaf, comparing it with green tea ( Camellia sinensis). For these purposes, methanolic extracts were prepared and phenolics content of quince leaf was determined by HPLC/UV. The antioxidant properties were assessed by Folin–Ciocalteu reducing capacity assay and by the ability to quench the stable free radical 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and to inhibit the 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative hemolysis of human erythrocytes. 5- O-Caffeoylquinic acid was found to be the major phenolic compound in quince leaf extract. Quince leaf exhibited a significantly higher reducing power than green tea (mean value of 227.8 ± 34.9 and 112.5 ± 1.5 g/kg dry leaf, respectively). Quince leaf extracts showed similar DPPH radical-scavenging activities (EC 50 mean value of 21.6 ± 3.5 μg/ml) but significantly lower than that presented by green tea extract (EC 50 mean value of 12.7 ± 0.1 μg/ml). Under the oxidative action of AAPH, quince leaf methanolic extract significantly protected the erythrocyte membrane from hemolysis in a similar manner to that found for green tea (IC 50 mean value of 30.7 ± 6.7 and 24.3 ± 9.6 μg/ml, respectively, P > 0.05). These results point that quince leaf may have application as preventive or therapeutic agent in diseases in which free radicals are involved.