Details

Autor(en) / Beteiligte

• Esquifino, Ana I.
• Chacon, Fernando
• Cano, Pilar
• Marcos, Ascensión
• Cutrera, Rodolfo A.
• Cardinali, Daniel P.

Titel

Twenty-four-hour rhythms of mitogenic responses, lymphocyte subset populations and amino acid content in submaxillary lymph nodes of growing male rats subjected to calorie restriction

Ist Teil von

• Journal of neuroimmunology, 2004-11, Vol.156 (1), p.66-73

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2004

Quelle

MEDLINE

Beschreibungen/Notizen

• Peripubertal male Wistar rats were submitted to a calorie restriction diet enriched in proteins and low in fat and carbohydrates for 4 weeks, and starting on day 35 of life. Mitogenic responses, lymphocyte subset populations, interferon (IFN)- γ release and amino acid content were determined in submaxillary lymph nodes at six time intervals during the 24-h span. The diet employed completely arrested growth in growing rats. After caloric restriction, mean values of Con A response, lymph node T and CD4 + cell number and CD4 +/CD8 + ratio augmented, whereas those of B cell number, IFN-γ release and glutamine and glutamate concentration decreased. Calorie restriction modified 24-h rhythmicity of lymph node mitogenic responses to Con A and LPS, and of T, T–B, CD4 + and CD4 +–CD8 + lymph node cell subsets. It also changed the 24-h pattern of lymph node IFN-γ release and glutamine, aspartate, glutamate and taurine lymph node content. Availability of nutrients presumably affects the mechanisms that modulate the circadian variation of immune responsiveness in growing rats.