Plant and cell physiology, 2009-02, Vol.50 (2), p.423-428
2009
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Increase in ascorbate content of transgenic tobacco plants overexpressing the acerola (Malpighia glabra) phosphomannomutase gene
- Ist Teil von
- Plant and cell physiology, 2009-02, Vol.50 (2), p.423-428
- Ort / Verlag
- Japan: Oxford University Press
- Erscheinungsjahr
- 2009
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Phosphomannomutase (PMM; EC 5.4.2.8) catalyzes the interconversion of mannose-6-phosphate to mannose-1-phosphate in the Smirnoff-Wheeler pathway for the biosynthesis of L-ascorbic acid (AsA). We have cloned the PMM cDNA from acerola (Malpighia glabra), a plant containing an enormous amount of AsA. The AsA contents correlate with the PMM gene expression of the ripening fruits and leaves. The PMM activities in the leaves of acerola, tomato and Arabidopsis correlate with their respective AsA contents. Transgenic tobacco plants overexpressing the acerola PMM gene showed about a 2-fold increase in AsA contents compared with the wild type, with a corresponding correlation with the PMM transcript levels and activities.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0032-0781eISSN: 1471-9053DOI: 10.1093/pcp/pcn206Titel-ID: cdi_proquest_miscellaneous_66930569
- Format
- –
- Schlagworte
- Acerola (Malpighia glabra), ACIDE ASCORBIQUE, ACIDO ASCORBICO, Amino Acid Sequence, Arabidopsis, ASCORBIC ACID, Ascorbic Acid - metabolism, BIOSINTESIS, BIOSYNTHESE, BIOSYNTHESIS, CLONACION MOLECULAR, CLONAGE MOLECULAIRE, Cloning, Molecular, Enzyme activity, Fruit - genetics, Fruit - metabolism, GENE, Gene Expression Regulation, Plant, GENES, Genes, Plant, Lycopersicon esculentum, MALPIGHIA GLABRA, Malpighiaceae - genetics, Malpighiaceae - metabolism, MOLECULAR CLONING, Molecular Sequence Data, Nicotiana - genetics, Nicotiana - metabolism, NICOTIANA TABACUM, Phosphomannomutase, Phosphomannose isomerase, Phosphotransferases (Phosphomutases) - genetics, Phosphotransferases (Phosphomutases) - metabolism, Plant Proteins - genetics, Plant Proteins - metabolism, PLANTAS TRANSGENICAS, PLANTE TRANSGENIQUE, Plants, Genetically Modified - genetics, Plants, Genetically Modified - metabolism, RNA, Plant - genetics, Sequence Alignment, TRANSGENIC PLANTS, Transgenic tobacco