Details

Autor(en) / Beteiligte

• Ohba, Kazuki
• Kubo, Shoji
• Tamori, Akihiro
• Hirohashi, Kazuhiro
• Tanaka, Hiromu
• Shuto, Taichi
• Nishiguchi, Shuhei
• Kinoshita, Hiroaki

Titel

Previous or occult hepatitis B virus infection in hepatitis B surface antigen-negative and anti-hepatitis C-negative patients with hepatocellular carcinoma

Ist Teil von

• Surgery today (Tokyo, Japan), 2004-10, Vol.34 (10), p.842-848

Ort / Verlag

Japan

Erscheinungsjahr

2004

Quelle

MEDLINE

Beschreibungen/Notizen

• We investigated the clinical and virologic findings in hepatitis B surface antigen (HBsAg)-negative and anti-hepatitis C virus antibody (anti-HCV)-negative patients with hepatocellular carcinoma (HCC) to investigate the role of previous or occult hepatitis B virus (HBV) infections in the development of HCC. We examined sera and HCC samples from 40 HBsAg-negative and anti-HCV-negative patients. Sera were tested for some viral markers, and genomic DNA was extracted from the HCC samples. HBx RNA was also extracted from the HCC and amplified by a polymerase chain reaction with reverse transcription (RT-PCR). Hepatocellular carcinomas from five patients with anti-HBc (group 1, 25 patients) and nine patients without anti-HBc (group 2, 15 patients) were examined for HBx RNA. HBx RNA was detected in four of the five HCC samples from group 1 and in four of the nine HCC samples from group 2. These findings suggested that previous or occult hepatitis B virus infection is common in HBsAg-negative and anti-HCV-negative patients with HCC.