International immunology, 2004-10, Vol.16 (10), p.1467-1475
2004
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- Autor(en) / Beteiligte
- Titel
- Interaction of endotoxins with Toll-like receptor 4 correlates with their endotoxic potential and may explain the proinflammatory effect of Brucella spp. LPS
- Ist Teil von
- International immunology, 2004-10, Vol.16 (10), p.1467-1475
- Ort / Verlag
- England: Oxford University Press
- Erscheinungsjahr
- 2004
- Quelle
- Oxford Journals 2020 Medicine
- Beschreibungen/Notizen
- Endotoxins displaying differences in the chemical structure of their lipid A were used to induce the expression of chemokines in the human monocytic THP-1 cell line. LPS from two enterobacterial species such as Escherichia coli and Yersinia enterocolitica induced mRNA expression of IFN-γ-inducible protein (IP)-10, macrophage-inflammatory protein (MIP)-1α, MIP-1β, monocyte chemoattractant protein (MCP)-1 and IL-8. LPS from the non-enterobacterial genera Brucella and Ochrobactrum induced the expression of these chemokines to a lower extent. Attempts to address the signaling routes involved in these responses were carried out in transiently transfected HEK293 cells. Induction of κB-driven transcriptional activity by enterobacterial LPS was observed in cells transfected with TLR-4 alone, although co-transfection of TLR-4, MD-2 and CD14 provided optimal induction. The response to Brucella spp. and Ochrobactrum anthropi LPS was only significant at the concentration of 10 μg/ml. These data indicate that LPS from Brucella spp. and O. anthropi, which contain lipid A moieties with structural features different from those of Enterobacteriaceae elicit biochemical signaling via TLR-4 only at high concentrations. Neither TLR-1, TLR-2 and TLR-6 nor heterodimeric combinations of these receptor molecules are involved. Conversely, the ability of LPS to activate the TLR-4 route is a reliable molecular biomarker for endotoxicity.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0953-8178, 1460-2377eISSN: 1460-2377DOI: 10.1093/intimm/dxh148Titel-ID: cdi_proquest_miscellaneous_66891211
- Format
- –
- Schlagworte
- Animals, Antigens, Surface - immunology, Antigens, Surface - metabolism, bacterial infection, Brucella, Brucella - immunology, Carrier Proteins - immunology, Carrier Proteins - metabolism, Cell Line, chemokines, Chemokines - immunology, COX-2 cyclooxygenase-2, endotoxin shock, Endotoxins - immunology, Endotoxins - metabolism, Enterobacteriaceae, Escherichia coli, Gene Expression - drug effects, Humans, IP-10 IFN-γ-inducible protein, KDO 2-keto-3-deoxyoctulosonic acid, LBP lipopolysaccharide-binding protein, Lipid A - immunology, lipopolysaccharide, Lipopolysaccharide Receptors - immunology, Lipopolysaccharide Receptors - metabolism, Lipopolysaccharides - chemistry, Lipopolysaccharides - immunology, Lipopolysaccharides - pharmacology, Lymphocyte Antigen 96, MD-2 myeloid differentiation protein-2, Membrane Glycoproteins - genetics, Membrane Glycoproteins - immunology, Membrane Glycoproteins - metabolism, MIP-1α macrophage-inflammatory protein-1α, MIP-1β macrophage-inflammatory protein-1β, Monocytes - immunology, NF-κB nuclear factor κB, normal T cell expressed and secreted, NOS-2 nitric oxide synthase-2, Ochrobactrum - immunology, Ochrobactrum anthropi, RANTES regulated upon activation, Receptors, Cell Surface - genetics, Receptors, Cell Surface - immunology, Receptors, Cell Surface - metabolism, RNA, Messenger - analysis, S-LPS Brucella smooth LPS, TLR Toll-like receptor, Toll-Like Receptor 1, Toll-Like Receptor 2, Toll-Like Receptor 4, Toll-Like Receptors, transcription factors, Transcriptional Activation, Transfection, Yersinia enterocolitica