Details

Autor(en) / Beteiligte

• Chen, Juan
• Stefflova, Klara
• Niedre, Mark J
• Wilson, Brian C
• Chance, Britton
• Glickson, Jerry D
• Zheng, Gang

Titel

Protease-Triggered Photosensitizing Beacon Based on Singlet Oxygen Quenching and Activation

Ist Teil von

• Journal of the American Chemical Society, 2004-09, Vol.126 (37), p.11450-11451

Ort / Verlag

Washington, DC: American Chemical Society

Erscheinungsjahr

2004

Quelle

MEDLINE

Beschreibungen/Notizen

• We report a new concept for type-II photosensitization, based on incorporating the photosensitizer (PS) and a singlet-oxygen (1O2) quenching/scavenging molecule onto a disease-targeting linker, such that the PS becomes activatable by light only when targeting has occurred. In this first proof-of-concept report, a model photosensitizing beacon was synthesized containing a pyropheophorbide as the PS and a carotenoid as the 1O2 quencher. These were kept in close proximity by the self-folding of a caspase-3-specific peptide sequence. Upon caspase-3-induced cleavage, the 1O2 production increased markedly, as measured directly by 1O2 near-infrared luminescence and lifetime measurements.