Details

Autor(en) / Beteiligte

• Webb, Tom R
• Chan, Li
• Cooray, Sadani N
• Cheetham, Michael E
• Chapple, J. Paul
• Clark, Adrian J. L

Titel

Distinct Melanocortin 2 Receptor Accessory Protein Domains Are Required for Melanocortin 2 Receptor Interaction and Promotion of Receptor Trafficking

Ist Teil von

• Endocrinology (Philadelphia), 2009-02, Vol.150 (2), p.720-726

Ort / Verlag

Chevy Chase, MD: Endocrine Society

Erscheinungsjahr

2009

Link zum Volltext

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• Melanocortin 2 receptor (MC2R) is the receptor for the pituitary hormone ACTH. When activated, MC2R stimulates cAMP production and adrenal steroidogenesis. The functional expression of the receptor requires melanocortin 2 receptor accessory protein (MRAP), a single-transmembrane domain protein involved in the trafficking of MC2R from the endoplasmic reticulum to the cell surface. Mutations in both MC2R and MRAP cause the inherited disease familial glucocorticoid deficiency. At present, little is known regarding the mechanism of MRAP in MC2R functional expression. Here we report the characterization of MRAP in the trafficking of MC2R to the cell surface and the formation of a functional receptor. We identify the transmembrane domain of MRAP as the MC2R interaction domain and a conserved N-terminal tyrosine-rich domain of MRAP that is required for trafficking MC2R to the cell surface. Melanocortin 2 receptor accessory protein interacts with the melanocortin 2 receptor (MC2R) via its transmembrane domain and requires a tyrosine-rich element for trafficking of the MC2R to the cell surface.