Tuberculosis (Edinburgh, Scotland), 2009-01, Vol.89 (1), p.22-28
2009
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- Autor(en) / Beteiligte
- Titel
- Mce2R from Mycobacterium tuberculosis represses the expression of the mce2 operon
- Ist Teil von
- Tuberculosis (Edinburgh, Scotland), 2009-01, Vol.89 (1), p.22-28
- Ort / Verlag
- Scotland: Elsevier Ltd
- Erscheinungsjahr
- 2009
- Quelle
- Access via ScienceDirect (Elsevier)
- Beschreibungen/Notizen
- Summary The mce2 operon is one of the four mce operons present in Mycobacterium tuberculosis that encode exported proteins with a probable role in the virulence mechanisms of this bacterium. In the present study we demonstrated that Rv0586 , which encodes a putative GntR-like regulator, is part of the mce2 operon. By using a promoter– lacZ fusion approach and bioinformatics tools, we found that Rv0586 represses the expression of Mce2 proteins and of a putative endonuclease IV, encoded by end ( Rv0670 ) gene. For this reason, we have re-named the repressor protein Mce2R. By gel-shift experiments Mce2R binding was determined to be located within the mce2 promoter region. In addition, two FadR-like operator motifs were identified within the promoter regions of both the mce2 operon and the end gene. These motifs overlap putative −10 and −35 promoter boxes. M. tuberculosis carrying mce2 and end promoter– lacZ fusions were used to infect J774 macrophage-like cells. Expression of β-galactosidase was induced after phagocytocis, suggesting that some cellular factor could be a key component of the molecular switch regulation expression of the mce2 operon. In conclusion, these results add novel evidence of the complex regulation of mce operon expression.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1472-9792eISSN: 1873-281XDOI: 10.1016/j.tube.2008.09.002Titel-ID: cdi_proquest_miscellaneous_66816104
- Format
- –
- Schlagworte
- Antigens, Bacterial - genetics, Bacterial Proteins - genetics, Binding Sites, Electrophoretic Mobility Shift Assay, Escherichia coli - genetics, Gene Expression, Gene Expression Regulation, Bacterial, Genes, Bacterial, GntR, Humans, Infectious Disease, mce2, Mycobacterium tuberculosis - genetics, Mycobacterium tuberculosis - metabolism, Mycobacterium tuberculosis - pathogenicity, Operator Regions, Genetic, Operon, Promoter Regions, Genetic, Protein Binding, Pulmonary/Respiratory, Regulation, Reporter, Repressor, Repressor Proteins - genetics, Reverse Transcriptase Polymerase Chain Reaction - methods, Transcription, Transcription, Genetic, Virulence - genetics