Details

Autor(en) / Beteiligte

• Martinez-Gamboa, Lorena
• Mei, Henrik
• Loddenkemper, Christoph
• Ballmer, Boris
• Hansen, Arne
• Lipsky, Peter E
• Emmerich, Florian
• Radbruch, Andreas
• Salama, Abdulgabar
• Dörner, Thomas

Titel

Role of the spleen in peripheral memory B-cell homeostasis in patients with autoimmune thrombocytopenia purpura

Ist Teil von

• Clinical immunology (Orlando, Fla.), 2009-02, Vol.130 (2), p.199-212

Ort / Verlag

Amsterdam: Elsevier Inc

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• Abstract The effect of splenectomy on circulating memory B cells in autoimmune thrombocytopenia purpura (AITP) patients has not yet been addressed. We therefore analyzed the distribution and phenotypic characteristics of B-cell subsets in non-splenectomized and splenectomized AITP patients and controls, as well as CD95 expression after B cell activation. Decreased frequencies of memory B cells in splenectomized individuals were observed, with a rapid decline of CD27+IgD+ and a slower decrease of CD27+IgD− and CD27−/IgD− cells. Similar results were noted following splenectomy in healthy donors (HD). CD95+ B cells were substantially increased in all subsets in patients with active AITP, indicating their enhanced activation status. After splenectomy, the percentage of CD95+ B cells were further increased in the CD27+IgD− post-switch memory population in AITP, but not in HD. CD95+CD27+ memory B cells largely reside in the region in the human spleen analogous to the murine marginal zone. Thus, the spleen plays a fundamental role in controlling peripheral memory B cell homeostasis in both AITP and HD and regulates activated CD95+ B cells in patients with AITP.