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United States: American Society for Biochemistry and Molecular Biology
Erscheinungsjahr
2004
Quelle
MEDLINE
Beschreibungen/Notizen
Previously, we demonstrated that antibodies printed on a solid support were able to detect protein-protein interaction in
mammalian cells. Here we further developed the antibody array system for detecting proteins with various post-translational
modifications in mammalian cells. In this novel approach, immunoprecipitated proteins were labeled with fluorescent dye followed
by incubation over antibody arrays. Targeted proteins, captured by the antibodies immobilized on PVDF membrane or glass slide,
were detected by means of near infrared fluorescent scanner or fluorescent microscopy. To demonstrate the application of the
antibody arrays in protein post-translational modifications, we profiled protein tyrosine phosphorylation, ubiquitination,
and acetylation in mammalian cells under different conditions. Our results indicate that antibody array technology can provide
a powerful means of profiling a large number of proteins with different post-translational modifications in cells.