Vaccine, 2009-01, Vol.27 (4), p.542-548
2009
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- Autor(en) / Beteiligte
- Titel
- Immunoproteomic analysis of Bordetella pertussis and identification of new immunogenic proteins
- Ist Teil von
- Vaccine, 2009-01, Vol.27 (4), p.542-548
- Ort / Verlag
- Kidlington: Elsevier Ltd
- Erscheinungsjahr
- 2009
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Abstract Bordetella pertussis is the causative agent of highly communicable respiratory infection whooping cough (pertussis) which remains one of the world's leading causes of vaccine-preventable deaths. In the present study, total soluble proteins extracted from two B. pertussis strains, Tohama I and the local isolate Saadet were separated by two-dimensional gel electrophoresis and analyzed by Western blotting for their reactivity with the antisera obtained from the mice immunized with inactivated whole cells as well as those collected from the mice challenged intraperitoneally with live cells of each strain. Of a total of 25 immunogenic proteins identified, 21 were shown to be the novel antigens for B. pertussis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0264-410XeISSN: 1873-2518DOI: 10.1016/j.vaccine.2008.11.020Titel-ID: cdi_proquest_miscellaneous_66779892
- Format
- –
- Schlagworte
- Allergy and Immunology, Antibodies, Bacterial - immunology, Antigens, Bacterial - analysis, Applied microbiology, Bacterial Proteins - analysis, Bacterial Proteins - genetics, Bacterial Proteins - immunology, Bacteriology, Biological and medical sciences, Bordetella pertussis, Bordetella pertussis - chemistry, Bordetella pertussis - immunology, Bordetella pertussis - isolation & purification, Electrophoresis, Gel, Two-Dimensional, Fatalities, Fundamental and applied biological sciences. Psychology, Immunization, Immunoproteomics, Microbiology, Miscellaneous, Pathogens, Peptides, Preventable deaths, Protein Sorting Signals, Proteins, Proteome, Proteomics, Proteomics - methods, Vaccine candidates, Vaccines, Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects), Whooping cough