Science (American Association for the Advancement of Science), 2004-08, Vol.305 (5685), p.858-862
2004
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Details
- Autor(en) / Beteiligte
- Titel
- Structural Basis of Mitochondrial Tethering by Mitofusin Complexes
- Ist Teil von
- Science (American Association for the Advancement of Science), 2004-08, Vol.305 (5685), p.858-862
- Ort / Verlag
- Washington, DC: American Association for the Advancement of Science
- Erscheinungsjahr
- 2004
- Quelle
- American Association for the Advancement of Science EJournals
- Beschreibungen/Notizen
- Vesicle fusion involves vesicle tethering, docking, and membrane merger. We show that mitofusin, an integral mitochondrial membrane protein, is required on adjacent mitochondria to mediate fusion, which indicates that mitofusin complexes act in trans (that is, between adjacent mitochondria). A heptad repeat region (HR2) mediates mitofusin oligomerization by assembling a dimeric, antiparallel coiled coil. The transmembrane segments are located at opposite ends of the 95 angstrom coiled coil and provide a mechanism for organelle tethering. Consistent with this proposal, truncated mitofusin, in an HR2-dependent manner, causes mitochondria to become apposed with a uniform gap. Our results suggest that HR2 functions as a mitochondrial tether before fusion.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.1099793Titel-ID: cdi_proquest_miscellaneous_66771453
- Format
- –
- Schlagworte
- 3T3 cells, Amino Acid Sequence, Amino Acid Substitution, Animals, Biological and medical sciences, Biology, Cell fusion, Cell Line, Cell lines, Cell structures and functions, Crystallography, X-Ray, Dimerization, Evolutionary linguistics, Fundamental and applied biological sciences. Psychology, Fusion, GTP Phosphohydrolases - chemistry, GTP Phosphohydrolases - metabolism, Humans, Hybrid Cells, Hydrophobic and Hydrophilic Interactions, Intracellular Membranes - physiology, Intracellular Membranes - ultrastructure, Lymphocytes, Membrane Fusion, Membranes, Mice, Mitochondria, Mitochondria - metabolism, Mitochondria - ultrastructure, Mitochondria and cell respiration, Mitochondrial DNA, Models, Molecular, Molecular and cellular biology, Molecular Sequence Data, Mutation, NIH 3T3 cells, Organelles, P branes, Properties, Protein Structure, Secondary, Protein Structure, Tertiary, Proteins, Retroviridae, Topology