Applied and Environmental Microbiology, 2004-08, Vol.70 (8), p.5037-5040
2004
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- Autor(en) / Beteiligte
- Titel
- Direct production of ethanol from raw corn starch via fermentation by use of a novel surface-engineered yeast strain codisplaying glucoamylase and alpha-amylase
- Ist Teil von
- Applied and Environmental Microbiology, 2004-08, Vol.70 (8), p.5037-5040
- Ort / Verlag
- Washington, DC: American Society for Microbiology
- Erscheinungsjahr
- 2004
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Direct and efficient production of ethanol by fermentation from raw corn starch was achieved by using the yeast Saccharomyces cerevisiae codisplaying Rhizopus oryzae glucoamylase and Streptococcus bovis alpha-amylase by using the C-terminal-half region of alpha-agglutinin and the flocculation functional domain of Flo1p as the respective anchor proteins. In 72-h fermentation, this strain produced 61.8 g of ethanol/liter, with 86.5% of theoretical yield from raw corn starch.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0099-2240eISSN: 1098-5336DOI: 10.1128/AEM.70.8.5037-5040.2004Titel-ID: cdi_proquest_miscellaneous_66755859
- Format
- –
- Schlagworte
- alcoholic fermentation, alpha-amylase, alpha-Amylases - genetics, alpha-Amylases - metabolism, Biological and medical sciences, Biotechnology, corn starch, Culture Media, Ethanol, Ethanol - metabolism, ethanol production, Fermentation, Fundamental and applied biological sciences. Psychology, gene expression, Gene Expression Regulation, Fungal, gene transfer, Genetic Engineering - methods, genetically engineered microorganisms, glucan 1,4-alpha-glucosidase, Glucan 1,4-alpha-Glucosidase - genetics, Glucan 1,4-alpha-Glucosidase - metabolism, Industrial Microbiology - methods, Methods. Procedures. Technologies, Microbial engineering. Fermentation and microbial culture technology, Microbiology, Physiology and Biotechnology, Plasmids, Recombinant Proteins, Rhizopus - enzymology, Rhizopus - genetics, Rhizopus oryzae, Saccharomyces cerevisiae, Saccharomyces cerevisiae - enzymology, Saccharomyces cerevisiae - genetics, Starch - metabolism, Streptococcus bovis, Streptococcus bovis - enzymology, Streptococcus bovis - genetics, Zea mays - chemistry, Zea mays - metabolism