Details

Autor(en) / Beteiligte

• Dinant, S.
• Roseboom, H. J.
• Levi, M.
• van Vliet, A. K.
• van Gulik, T. M.

Titel

Hypothermic in situ perfusion of the porcine liver using Celsior or Ringer-lactate solution

Ist Teil von

• Langenbeck's archives of surgery, 2009, Vol.394 (1), p.143-150

Ort / Verlag

Berlin/Heidelberg: Springer-Verlag

Erscheinungsjahr

2009

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• Background Hypothermic perfusion (HP) of the liver is applied during total vascular exclusion (TVE) to reduce ischemic injury during liver resection. No studies have been performed comparing different perfusion solutions for HP. The aim of this experimental study was to compare Ringer-lactate solution (RL) with Celsior solution (Cs) for HP in a pig model of 60-min TVE. Method Twenty pigs underwent 60-min TVE of the liver. Groups were TVE without HP (no-HP, n  = 9), TVE with HP using RL ( n  = 6), and TVE with HP using Cs ( n  = 5). Blood and liver tissue samples were taken before TVE and during 24-h reperfusion. Results In the no-HP group, plasma aspartate aminotransferase values were significantly increased during reperfusion ( p  < 0.05), while liver tissue pO 2 levels ( p  < 0.01) were decreased when compared to the HP groups. After 24-h reperfusion, bile production and liver tissue glutathione content were significantly higher ( p  < 0.05) in the Cs group (42.0 ± 1.7 mL/h and 44.9 ± 2.2 nmol/mg, respectively) as compared to the RL group (31.5 ± 3.5 mL/h and 19.6 ± 1.8 nmol/mg, respectively). Conclusion The protective effect of HP during TVE was confirmed in this study. HP with Cs was more effective in reducing ischemic injury as compared to HP with RL.