Details

Autor(en) / Beteiligte

• da Fonseca, Patrícia
• de Freitas, Luis Alexandre Pedro
• Pinto, Luis Felipe Ribeiro
• Pestana, Cezar Rangel
• Bonato, Pierina Sueli

Titel

Enantioselective analysis of oxybutynin and N-desethyloxybutynin with application to an in vitro biotransformation study

Ist Teil von

• Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2008-11, Vol.875 (1), p.161-167

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2008

Quelle

MEDLINE

Beschreibungen/Notizen

• An enantioselective method using liquid-phase microextraction (LPME) followed by HPLC analysis was developed for the determination of oxybutynin (OXY) and its major metabolite N-desethyloxybutynin (DEO) in rat liver microsomal fraction. The LPME procedure was optimized using multifactorial experiments. Under the optimal extraction conditions, the mean recoveries were 61 and 55% for ( R)-OXY and ( S)-OXY, respectively, and 70 and 76% for ( R)-DEO and ( S)-DEO, respectively. The validated method was employed to an in vitro biotransformation study using rat liver microsomal fraction. The results demonstrated the enantioselective biotransformation of OXY.