Carbohydrate research, 2004-07, Vol.339 (10), p.1727-1737
2004
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Details
- Autor(en) / Beteiligte
- Titel
- Synthesis and characterisation of novel chromogenic substrates for human pancreatic α-amylase
- Ist Teil von
- Carbohydrate research, 2004-07, Vol.339 (10), p.1727-1737
- Ort / Verlag
- Netherlands: Elsevier Ltd
- Erscheinungsjahr
- 2004
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Graphic Derivatives of maltose and maltotriose were chemically synthesised as substrates for human pancreatic α-amylases and subjected to kinetic analysis. Rates measured were shown to reflect both hydrolysis and transglycosylation reactions. 4- O-Methylated derivatives of these substrates underwent only hydrolysis, thereby simplifying kinetic analyses. These modified substrates may be used for the detection and kinetic analysis of α-amylases, and are useful in rapidly screening for novel α-amylase inhibitors and for subsequent kinetic characterisation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0008-6215eISSN: 1873-426XDOI: 10.1016/j.carres.2004.05.019Titel-ID: cdi_proquest_miscellaneous_66659756
- Format
- –
- Schlagworte
- alpha-Amylases - antagonists & inhibitors, alpha-Amylases - chemistry, Biochemistry - methods, Chromatography, High Pressure Liquid, Chromogenic substrates, Enzyme Inhibitors - pharmacology, Glycosidase, Glycosylation, Human pancreatic α-amylase, Humans, Hydrogen-Ion Concentration, Hydrolysis, Kinetics, Models, Chemical, Pancreas - enzymology, Spectrophotometry, Substrate Specificity, Temperature, Time Factors, Ultraviolet Rays, α-Amylase inhibitors