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Tetranucleotide Microsatellite Instability in Surgical Margins for Prediction of Local Recurrence of Head and Neck Squamous Cell Carcinoma
Ist Teil von
Clinical cancer research, 2004-06, Vol.10 (12), p.4022-4028
Ort / Verlag
Philadelphia, PA: American Association for Cancer Research
Erscheinungsjahr
2004
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
Purpose: Postoperative radiotherapy is used to prevent local recurrence of head and neck squamous cell carcinoma in patients with
positive surgical margins. We sought to determine whether tetranucleotide microsatellite instability could be detected in
surgical margins and used to predict local recurrence.
Experimental Design: We prospectively collected tumor and surgical margin specimens from patients with head and neck squamous cell carcinoma who
had undergone surgical resection at Institut Gustave-Roussy during a 1-year period. Margins were considered positive if extensive
pathological examination revealed either carcinoma within 5 mm or dysplasia. We tested five tetranucleotide microsatellite
markers (UT5085, L17686, D9S753, ACTBP2, and CSF1R) in the tumor specimens and paired surgical margins of the patients whose
margins were negative on pathological examination.
Results: Pathological examination revealed that among the 76 patients, 22 had positive margins; therefore, these patients were excluded.
Of the 54 remaining patients, 26 (48%) had tumors informative for markers UT5085, L17686, or both; the other 3 markers were
not informative. Seven (27%) of the 26 informative tumors had the same instability pattern in the surgical margins. At a median
follow-up of 26 months, 5 of the 7 local recurrences occurred in patients with molecularly positive surgical margins. A strong,
independent association was found between positive surgical margins and local recurrence ( P = 0.01; hazard ratio, 6.49).
Conclusions: Tetranucleotide microsatellite instability in surgical margins may be a useful biomarker to predict local recurrence of head
and neck squamous cell carcinoma in patients with apparently disease-free margins.