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Chlorophyll bleaching by UV-irradiation in vitro and in situ: Absorption and fluorescence studies
Ist Teil von
Radiation physics and chemistry (Oxford, England : 1993), 2009, Vol.78 (1), p.25-32
Ort / Verlag
Elsevier Ltd
Erscheinungsjahr
2009
Quelle
Access via ScienceDirect (Elsevier)
Beschreibungen/Notizen
Chlorophyll bleaching by UV-irradiation has been studied by absorbance and fluorescence spectroscopy in extracts containing mixtures of photosynthetic pigments, in acetone and
n-hexane solutions, and in aqueous thylakoid suspensions. Chlorophyll undergoes destruction (bleaching) accompanied by fluorescent transient formation obeying first-order kinetics. The bleaching is governed by UV-photon energy input, as well as by different chlorophyll molecular organizations in solvents of different polarities (
in vitro), and in thylakoids (
in situ). UV-C-induced bleaching of chlorophylls in thylakoids is probably caused by different mechanisms compared to UV-A- and UV-B-induced bleaching.