Details

Autor(en) / Beteiligte

• Kim, Deok-Ho
• Seo, Chang-Ho
• Han, Karam
• Kwon, Keon Woo
• Levchenko, Andre
• Suh, Kahp-Yang

Titel

Guided Cell Migration on Microtextured Substrates with Variable Local Density and Anisotropy

Ist Teil von

• Advanced functional materials, 2009-05, Vol.19 (10), p.1579-1586

Ort / Verlag

Weinheim: WILEY-VCH Verlag

Erscheinungsjahr

2009

Quelle

Wiley Online Library All Journals

Beschreibungen/Notizen

• This work reports the design of and experimentation with a topographically patterned cell culture substrate of variable local density and anisotropy as a facile and efficient platform to guide the organization and migration of cells in spatially desirable patterns. Using UV‐assisted capillary force lithography, an optically transparent microstructured layer of a UV curable poly(urethane acrylate) resin is fabricated and employed as a cell‐culture substrate after coating with fibronectin. With variable local pattern density and anisotropy present in a single cell‐culture substrate, the differential polarization of cell morphology and movement in a single experiment is quantitatively characterized. It is found that cell shape and velocity are exquisitely sensitive to variation in the local anisotropy of the two‐dimensional rectangular lattice arrays, with cell elongation and speed decreasing on symmetric lattice patterns. It is also found that cells could integrate orthogonal spatial cues when determining the direction of cell orientation and movement. Furthermore, cells preferentially migrate toward the topographically denser areas from sparser ones. Consistent with these results, it is demonstrated that systematic variation of local densities of rectangular lattice arrays enable a planar assembly of cells into a specified location. It is envisioned that lithographically defined substrates of variable local density and anisotropy not only provide a new route to tailoring the cell‐material interface but could serve as a template for advanced tissue engineering. Microtextured substrates with variable local density and anisotropy (see image) are designed to guide the organization and migration of fibroblasts in spatially desirable locations. Cell motility is sensitive to variation in the local density and anisotropy of rectangular lattice, with cell elongation and speed decreasing on symmetric lattice. Also, cells integrate orthogonal spatial cues when determining the direction of their orientation and movement.