Details

Autor(en) / Beteiligte

• XU, Ying-Ming
• PAN, Hong-Qing
• WU, San-Hua
• ZHANG, Bai-Lin

Titel

Interaction Between Tripeptide Gly-Gly-His and Cu2+ Probed by Microcantilevers

Ist Teil von

• Chinese Journal of Analytical Chemistry, 2009-06, Vol.37 (6), p.783-787

Erscheinungsjahr

2009

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• Gly-Gly-His tripeptide modified microcantilever was developed by carbodiimide attachment of the Gly-Gly-His tripeptide onto a 3-mercaptopropionic acid (MPA) modified gold surface. The interaction of peptide with Cu(2+) ion was studied. At a relative high concentration of Cu(2+), the cantilever bent toward the gold side initially as the N atom of imidazole ring and carboxyl group in different peptide coordinate with Cu(2+), which results in a tensile surface stress. And then the reversed deflection of microcantilever was observed, which implies that the peptide-Cu(2+) complex are formed with conformation transition. In another case, i. e., at a relative low concentration of Cu(2+), only the process of conformation transition was observed due to the coordination mode can not be formed initially. The influences of pH and salt concentration of the test solution on the performance of the sensor were studied. The results show that the maximum deflection was obtained at pH 7 and the bonding of Cu(2+) to the Gly-Gly-His tripeptide was inhibited due to the formation of CuClx(2-x).