Details

Autor(en) / Beteiligte

• Shivarathri, Raju
• Chauhan, Manju
• Datta, Abhishek
• Das, Diprasom
• Karuli, Adela
• Aptekmann, Ariel
• Jenull, Sabrina
• Kuchler, Karl
• Thangamani, Shankar
• Chowdhary, Anuradha
• Desai, Jigar V
• Chauhan, Neeraj
• Chiller, Tom

Titel

The Candida auris Hog1 MAP kinase is essential for the colonization of murine skin and intradermal persistence

Ist Teil von

• mBio, 2024-10, p.e0274824

Ort / Verlag

United States: American Society for Microbiology

Erscheinungsjahr

2024

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• , a multidrug-resistant human fungal pathogen, was first identified in 2009 in Japan. Since then, systemic infections have now been reported in more than 50 countries, with mortality rates of 30%-60%. A major contributing factor to its high inter- and intrahospital clonal transmission is that unlike most species, displays unique skin tropism and can stay on human skin for a prolonged period. However, the molecular mechanisms responsible for skin colonization, intradermal persistence, and systemic virulence are poorly understood. Here, we report that Hog1 mitogen-activated protein kinase is essential for efficient skin colonization, intradermal persistence as well as systemic virulence. RNA-seq analysis of wild-type parental and Δ mutant strains revealed marked downregulation of genes involved in processes such as cell adhesion, cell wall rearrangement, and pathogenesis in Δ mutant compared to the wild-type parent. Consistent with these data, we found a prominent role for Hog1 in maintaining cell wall architecture, as the Δ mutant demonstrated a significant increase in cell-surface β-glucan exposure and a concomitant reduction in chitin content. Additionally, we observed that Hog1 was required for biofilm formation and fungal survival when challenged with primary murine macrophages and neutrophils . Collectively, these findings have important implications for understanding the skin adherence mechanisms and penetration of skin epithelial layers preceding bloodstream infections. is a World Health Organization fungal priority pathogen and an urgent public health threat recognized by the Centers for Disease Control and Prevention. has a unique ability to colonize human skin. It also persists on abiotic surfaces in healthcare environments for an extended period of time. These attributes facilitate the inter- and intrahospital clonal transmission of . Therefore, understanding skin colonization mechanisms is critical for infection control, especially in hospitals and nursing homes. However, despite its profound clinical relevance, the molecular and genetic basis of skin colonization mechanisms are poorly understood. Herein, we present data on the identification of the Hog1 MAP kinase as a key regulator of skin colonization. These findings lay the foundation for further characterization of unique mechanisms that promote fungal persistence on human skin.