Details

Autor(en) / Beteiligte

• Hu, Yunfei
• Tang, Yuchen
• Zhang, Jianyu
• Guo, Xuting
• Wang, Jiaru
• Zhang, Xinmeng
• Li, Zheng
• Yu, Hao
• Li, Wenlong

Titel

In vitro digestion and fermentation of polysaccharides from nine common Polygonatum spp. and their impact on human gut microbiota

Ist Teil von

• International journal of biological macromolecules, 2024-11, Vol.280 (Pt 4), p.136052, Article 136052

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2024

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• This study aimed to investigate the dynamic changes in the physicochemical properties of polysaccharides from nine common Polygonatum spp. during in vitro simulated saliva-gastrointestinal digestion, in vitro fermentation, and their subsequent effects on human gut microbiota. Results revealed that the total sugar contents of Polygonatum spp. polysaccharides almost had little changes during the vitro digestion, and the molecular weight presented a downward trend. The in vitro digestion process produced almost no free monosaccharide, with small variations on FT-IR spectroscopy analysis. However, during the in vitro fermentation process, the polysaccharides generated remarkable changes, the total sugar showed a downward trend, and the molecular weight was degraded. There were significant changes in the monosaccharide composition, and possibly the sugar occurred isomerism. Regarding the concentrations of short-chain fatty acids, both acetic acid and propionic acid were found to be significantly elevated in the treatment group compared to the control group, and the pH value dramatically decreased. Simultaneously, Polygonatum spp. polysaccharides could remarkably modulate the richness of microbial communities and improved their diversity, especially Narrowly Defined Clostridium, and Bacteroidetes. In general, this study can be helpful to better understand the potential digestion and fermentation mechanism of the genus Polygonatum polysaccharides. [Display omitted] •Polysaccharides from nine common Polygonatum spp. were not digested in simulated saliva, gastric and small intestinal•Polysaccharides from nine common Polygonatum spp. could reached large intestine and were utilized by intestinal microbiota•Fermentation of the polysaccharides from nine common Polygonatum spp. promoted the production of short chain fatty acids•Polysaccharides from nine common Polygonatum spp. enhanced the relative abundances of beneficial bacteria