Details

Autor(en) / Beteiligte

• Long, Lu
• Xu, Fu‐Chun
• Yuan, Man
• Shang, Shen‐Zhai
• Song, Hao‐Ge
• Zhao, Jing‐Ruo
• Hu, Gai‐Yuan
• Zhang, Zhen‐Nan
• Zhao, Xiao‐Tong
• Ma, Jia‐Yi
• Hussain, Amjad
• Wang, Ping
• Cai, Ying‐Fan
• Jin, Shuang‐Xia
• Gao, Wei

Titel

GhHAM regulates GoPGF‐dependent gland development and contributes to broad‐spectrum pest resistance in cotton

Ist Teil von

• The Plant journal : for cell and molecular biology, 2024-07, Vol.119 (2), p.879-894

Ort / Verlag

England: Blackwell Publishing Ltd

Erscheinungsjahr

2024

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• SUMMARY Cotton is a globally cultivated crop, producing 87% of the natural fiber used in the global textile industry. The pigment glands, unique to cotton and its relatives, serve as a defense structure against pests and pathogens. However, the molecular mechanism underlying gland formation and the specific role of pigment glands in cotton's pest defense are still not well understood. In this study, we cloned a gland‐related transcription factor GhHAM and generated the GhHAM knockout mutant using CRISPR/Cas9. Phenotypic observations, transcriptome analysis, and promoter‐binding experiments revealed that GhHAM binds to the promoter of GoPGF, regulating pigment gland formation in cotton's multiple organs via the GoPGF‐GhJUB1 module. The knockout of GhHAM significantly reduced gossypol production and increased cotton's susceptibility to pests in the field. Feeding assays demonstrated that more than 80% of the cotton bollworm larvae preferred ghham over the wild type. Furthermore, the ghham mutants displayed shorter cell length and decreased gibberellins (GA) production in the stem. Exogenous application of GA3 restored stem cell elongation but not gland formation, thereby indicating that GhHAM controls gland morphogenesis independently of GA. Our study sheds light on the functional differentiation of HAM proteins among plant species, highlights the significant role of pigment glands in influencing pest feeding preference, and provides a theoretical basis for breeding pest‐resistant cotton varieties to address the challenges posed by frequent outbreaks of pests. Significance Statement Pigment glands are essential for cotton's pest defense, but their formation mechanisms remained unclear. We demonstrated that the GhHAM transcription factor, through the GoPGF‐GhJUB1 pathway, is pivotal for gland development and pest resistance, offering new insights into breeding pest‐resistant cotton.