Details

Autor(en) / Beteiligte

• Shi, Shan-Shan
• Li, Xiao-Jian
• Ma, Rong-Na
• Shang, Lei
• Zhang, Wei
• Zhao, Huai-Qing
• Jia, Li-Ping
• Wang, Huai-Sheng

Titel

A novel dual-signal output strategy for POCT of CEA based on a smartphone electrochemical aptasensing platform

Ist Teil von

• Mikrochimica acta (1966), 2024-07, Vol.191 (7), p.407, Article 407

Ort / Verlag

Vienna: Springer Vienna

Erscheinungsjahr

2024

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A smartphone-based electrochemical aptasensing platform was developed for the point-of-care testing (POCT) of carcinoembryonic antigen (CEA) based on the ferrocene (Fc) and PdPt@PCN-224 dual-signal labeled strategy. The prepared PdPt@PCN-224 nanocomposite showed a strong catalytic property for the reduction of H 2 O 2 . Phosphate group-labeled aptamer could capture PdPt@PCN-224 by Zr–O–P bonds to form PdPt@PCN-224-P-Apt. Therefore, a dual signal labeled probe was formed by the hybridization between Fc-DNA and PdPt@PCN-224-P-Apt. The presence of CEA forced PdPt@PCN-224-P-Apt to leave the electrode surface due to the specific affinity, leading to the decrease of the reduction current of H 2 O 2 . At the same time, the Fc-DNA strand changed to hairpin structure, which made Fc closer to the electrode and resulted in the increase of the oxidation current of Fc. Thus, CEA can be accurately determined through both signals: the decrease of H 2 O 2 reduction current and the increase of Fc oxidation current, which could avoid the false positive signal. Under the optimal conditions, the prepared aptasensor exhibited a wide linear range from 1 pg·mL −1 to 100 ng·mL −1 and low detection limits of 0.98 pg·mL −1 and 0.27 pg·mL −1 with Fc and PdPt@PCN-224 as signal labels, respectively. The aptasensor developed in this study has successfully demonstrated its capability to detect CEA in real human serum samples. These findings suggest that the proposed sensing platform will hold great potential for clinical tumor diagnosis and monitoring. Graphical abstract