Details

Autor(en) / Beteiligte

• Kang, Kang
• Sun, Chuannan
• Li, Hui
• Liu, Xiaojia
• Deng, Jingyuan
• Chen, Silei
• Zeng, Le
• Chen, Jiahao
• Liu, Xinyi
• Kuang, Jiahao
• Xiang, Jingjing
• Cheng, Jingqian
• Liao, Xiaoyun
• Lin, Mujin
• Zhang, Xingshi
• Zhan, Chuzhi
• Liu, Sisi
• Wang, Jun
• Niu, Yanqin
• Liu, Cuilian
• Liang, Cai
• Zhu, Jinsheng
• Liang, Shuxin
• Tang, Haiyang
• Gou, Deming

Titel

N6-methyladenosine-driven miR-143/145-KLF4 circuit orchestrates the phenotypic switch of pulmonary artery smooth muscle cells

Ist Teil von

• Cellular and molecular life sciences : CMLS, 2024-12, Vol.81 (1), p.256

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2024

Link zum Volltext

Quelle

SpringerNature Journals

Beschreibungen/Notizen

• Pulmonary hypertension (PH) is characterized by vascular remodeling predominantly driven by a phenotypic switching in pulmonary artery smooth muscle cells (PASMCs). However, the underlying mechanisms for this phenotypic alteration remain incompletely understood. Here, we identified that RNA methyltransferase METTL3 is significantly elevated in the lungs of hypoxic PH (HPH) mice and rats, as well as in the pulmonary arteries (PAs) of HPH rats. Targeted deletion of Mettl3 in smooth muscle cells exacerbated hemodynamic consequences of hypoxia-induced PH and accelerated pulmonary vascular remodeling in vivo. Additionally, the absence of METTL3 markedly induced phenotypic switching in PASMCs in vitro. Mechanistically, METTL3 depletion attenuated m 6 A modification and hindered the processing of pri-miR-143/145, leading to a downregulation of miR-143-3p and miR-145-5p. Inhibition of hnRNPA2B1, an m 6 A mediator involved in miRNA maturation, similarly resulted in a significant reduction of miR-143-3p and miR-145-5p. We demonstrated that miR-145-5p targets Krüppel-like factor 4 (KLF4) and miR-143-3p targets fascin actin-bundling protein 1 (FSCN1) in PASMCs. The decrease of miR-145-5p subsequently induced an upregulation of KLF4, which in turn suppressed miR-143/145 transcription, establishing a positive feedback circuit between KLF4 and miR-143/145. This regulatory circuit facilitates the persistent suppression of contractile marker genes, thereby sustaining PASMC phenotypic switch. Collectively, hypoxia-induced upregulation of METTL3, along with m 6 A mediated regulation of miR-143/145, might serve as a protective mechanism against phenotypic switch of PASMCs. Our results highlight a potential therapeutic strategy targeting m 6 A modified miR-143/145-KLF4 loop in the treatment of PH.