Details

Autor(en) / Beteiligte

• Kai, Mingxuan
• Shen, Wei-Ting
• Yu, Yiyan
• Wang, Dan
• Zhang, Jiayuan Alex
• Wang, Shuyan
• Fang, Ronnie H.
• Gao, Weiwei
• Zhang, Liangfang

Titel

Dual-Modal Cellular Nanoparticles for Continuous Neurotoxin Detoxification

Ist Teil von

• Nano letters, 2024-06, Vol.24 (24), p.7515-7523

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2024

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Neurotoxins are known for their extreme lethality. However, due to their enormous diversity, effective and broad-spectrum countermeasures are lacking. This study presents a dual-modal cellular nanoparticle (CNP) formulation engineered for continuous neurotoxin neutralization. The formulation involves encapsulating the metabolic enzyme N-sulfotransferase (SxtN) into metal–organic framework (MOF) nanoparticle cores and coating them with a natural neuronal membrane, termed “Neuron-MOF/SxtN-NPs”. The resulting nanoparticles combine membrane-enabled broad-spectrum neurotoxin neutralization with enzyme payload-enabled continuous neurotoxin neutralization. The studies confirm the protection of the enzyme payload by the MOF core and validate the continuous neutralization of saxitoxin (STX). In vivo studies conducted using a mouse model of STX intoxication reveal markedly improved survival rates compared with control groups. Furthermore, acute toxicity assessments show no adverse effects associated with the administration of Neuron-MOF/SxtN-NPs in healthy mice. Overall, Neuron-MOF/SxtN-NPs represent a unique biomimetic nanomedicine platform poised to effectively neutralize neurotoxins, marking an important advancement in the field of countermeasure nanomedicine.