Details

Autor(en) / Beteiligte

• Adiyeke, Esra
• Bakan, Nurten
• Uvez, Ayca
• Arslan, Devrim OZ
• Kilic, Sima
• Koc, Berkcan
• Ozer, Samed
• Saatci, Ozlem
• Armutak, Elif İlkay

Titel

The effect of N-acetylcysteine on the neurotoxicity of sevoflurane in developing hippocampus cells

Ist Teil von

• Neurotoxicology (Park Forest South), 2024-07, Vol.103, p.96-104

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2024

Link zum Volltext

Quelle

ScienceDirect

Beschreibungen/Notizen

• Sevoflurane, a common pediatric anesthetic, has been linked to neurodegeneration, raising safety concerns. This study explored N-acetylcysteine's protective potential against sevoflurane-induced neurotoxicity in rat hippocampi. Four groups were examined: Control: Received 6 hours of 3 l/min gas (air and 30 % O2) and intraperitoneal saline. NAC: Received 6 hours of 3 l/min gas and 150 mg/kg NAC intraperitoneally. Sev: Exposed to 6 hours of 3 l/min gas and 3 % sevoflurane. Sev+NAC: Received 6 hours of 3 l/min gas, 3 % sevoflurane, and 150 mg/kg NAC. Protein levels of NRF-2, NLRP3, IL-1β, caspase-1, Beclin 1, p62, LC3A, and apoptosis markers were assessed. Sevoflurane and NAC alone reduced autophagy, while Sev+NAC group maintained autophagy levels. Sev group had elevated NRF-2, NLRP3, pNRF2, Caspase-1, and IL-1β, which were reduced in Sev+NAC. Apoptosis was higher in Sev, but Sev+NAC showed reduced apoptosis compared to the control. In summary, sevoflurane induced neurotoxicity in developing hippocampus, which was mitigated by N-acetylcysteine administration. •Sevoflurane-induced neurotoxicity in developing brains involves disrupted autophagy, neuroinflammation, oxidative stress, and apoptosis, raising concerns for pediatric anesthesia.•N-acetylcysteine, with its antioxidant and anti-inflammatory properties, emerges as a potential protective agent against sevoflurane-induced neurotoxicity in a developing rat model.•The study shows that NAC preserves autophagy, suppresses neuroinflammation and oxidative stress, and mitigates apoptosis, offering a comprehensive approach to counter sevoflurane's harmful effects.•With its multifaceted protective effects, NAC shows promise in alleviating sevoflurane's neurotoxic impact on developing brain cells, providing valuable insights for clinical practice.